Biology
Dr
Peter Lockey directs Argenta’s comprehensive biology
capabilities, which include molecular biology, assay development,
and biochemical and cellular screening.
Our molecular biology capabilities cover cloning in a variety
of expression systems (E. coli, yeast, insect and
mammalian) as well as purification of proteins and membranes.
We are skilled in a range of assay methodologies, and can
develop and apply novel methodologies in addition to running
a client’s existing assay protocol, to ensure consistency
with historical data.
Our screening capabilities span biochemical and cell-based
functional assays for all enzyme, protein-protein or receptor
targets in a variety of techniques, including:
- Radioactivity (e.g. SPA, Flashplate, Cytostar T)
- Fluorescence (e.g. HTRF,FP, DELFIA, Molecular Devices
Flexstation)
- Absorbance
- Luminescence (e.g. ALPHA screen, reporter gene)
- Meso Scale electrochemiluminescence technology
A panel of profiling assays is also available to assess
selectivity versus family targets (e.g. in kinases) and also
inter-species selectivity.
We also develop and implement high-content assays in partnership
with TTP LabTech using the Acumen Explorer™ platform.
Functional assays can be performed in appropriate cell systems
to characterise the pharmacology and toxicity profile of lead
compounds.
For more information on our biology and screening capabilities
please contact us.
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Assay
development, biochemical and cellular screening
